|
KMID : 0043320100330101633
|
|
Archives of Pharmacal Research
2010 Volume.33 No. 10 p.1633 ~ p.1639
|
|
|
Site-directed mutagenesis of the toxin from the Chinese scorpion Buthus martensii Karsch (BmKAS): Insight into sites related to analgesic activity
|
|
Cui Yong
Song Yong-Bo
Ma Lin
Liu Yan Feng
Li Guo Dong
Wu Chun Fu
Zhang Jing Hai
|
|
|
Abstract
|
|
|
|
This study utilized the E. coli expression system to investigate the role of amino acid residues in toxin from the Chinese scorpion ? Buthus martensii Karsch (BmKAS). To evaluate the extent to which residues of the toxin core contribute to its analgesic activity, ten mutants of BmKAS were obtained by PCR. Using site-directed mutagenesis, all of these residues were substituted with different amino acids. This study represents a thorough mapping and elucidation of the epitopes that form the molecular basis of the toxin’s analgesic activity. Our results showed large mutant-dependent differences that emphasize the important roles of the studied residues.
|
|
|
KEYWORD
|
|
|
Analgesic activity, BmKAS, Homology modeling, Site directed mutagenesis, Structure-function relationship
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|